Hotspot

rbcL-accD

LSC · 60768-61540 · 773 bp

Back to Staphylea

Pi 0.00669

MarkerSeek score 64.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 10
pi: 0.00669
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ATTCCGCCTTAGACAACACC	55.0	50.0	58.0	57.9	906-921 bp	1.0	80.1
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	CGCCTTAGACAACACCGAAT	55.0	50.0	58.0	58.3	902-917 bp	1.0	78.8
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	GGGCTTTCGCTTATACTGGT	55.0	50.0	58.0	57.7	982-997 bp	1.0	78.3
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	ATTCCGCCTTAGACAACACC	55.0	50.0	57.7	57.9	892-907 bp	1.0	78.1
rbcL-accD_p5	GGCTAGCAAATGGAGTCCTG	ATTCCGCCTTAGACAACACC	55.0	50.0	58.3	57.9	904-919 bp	1.0	77.8