Hotspot

trnT-psbD

LSC · 34722-36118 · 1397 bp

Back to Staphylea

Pi 0.00597

MarkerSeek score 58.00

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1397
variable_sites: 16
pi: 0.00597
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	GACAAAAACGAAACGGTCCC	55.0	50.0	58.1	57.9	1548-1565 bp	1.0	79.8
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	GACAAAAACGAAACGGTCCC	50.0	50.0	58.1	57.9	1551-1568 bp	1.0	79.7
trnT-psbD_p3	GTGGTAGAGTAACGCCATGG	AACCGACAAAAACGAAACGG	55.0	45.0	58.1	57.8	1552-1569 bp	1.0	79.3
trnT-psbD_p4	TCAGTGGTAGAGTAACGCCA	AACCGACAAAAACGAAACGG	50.0	45.0	58.1	57.8	1555-1572 bp	1.0	79.1
trnT-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1547-1564 bp	1.0	78.8