Hotspot

trnE-trnT

LSC · 33765-34649 · 885 bp

Back to Staphylea

Pi 0.00871

MarkerSeek score 61.80

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 13
pi: 0.00871
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TTTCGTACTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	710-1026 bp	1.0	83.8
trnE-trnT_p2	TTTCGTACTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	689-1005 bp	1.0	83.3
trnE-trnT_p3	TTTCGTACTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	686-1002 bp	1.0	83.2
trnE-trnT_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	651-967 bp	1.0	82.9
trnE-trnT_p5	TTTCGTACTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	707-1023 bp	1.0	82.6