Hotspot

rpoB-trnC

LSC · 28677-29937 · 1261 bp

Back to Staphylea

Pi 0.00344

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1261
variable_sites: 8
pi: 0.00344
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	TCATTTCCATCCCCCAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	59.1	58.4	1325-1338 bp	1.0	69.5
rpoB-trnC_p2	TTCCCTCATTTCCATCCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	59.1	58.4	1330-1343 bp	1.0	69.5
rpoB-trnC_p3	ATTCCCTCATTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1331-1344 bp	1.0	69.4
rpoB-trnC_p4	CTCATTTCCATCCCCCAGC	AAAAGGATTTGCAGTCCCCC	57.9	50.0	57.9	58.4	1326-1339 bp	1.0	69.1
rpoB-trnC_p5	TCATTTCCATCCCCCAGCAT	GCGACACCCAGATTTGAACT	50.0	50.0	59.1	58.5	1350-1363 bp	1.0	68.6