Hotspot

atpH-atpI

LSC · 15321-16347 · 1027 bp

Back to Staphylea

Pi 0.00292

MarkerSeek score 61.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 6
pi: 0.00292
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.0	57.9	1188-1200 bp	1.0	79.7
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1141-1153 bp	1.0	79.7
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1154-1166 bp	1.0	79.7
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1107-1119 bp	1.0	79.7
atpH-atpI_p5	AATAGAAGCAAGCCCGACAG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1111-1123 bp	1.0	79.4