Hotspot

psbI-trnS-GCU

LSC · 9159-9544 · 386 bp

Back to Staphylea

Pi 0.025

MarkerSeek score 54.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 9
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.6	58.0	290-482 bp	1.0	82.9
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.6	58.0	291-483 bp	1.0	82.9
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.6	58.1	309-501 bp	1.0	82.0
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.6	58.4	288-480 bp	1.0	80.1
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.6	58.4	295-487 bp	1.0	79.9