Hotspot

rps16-trnQ

LSC · 6620-8116 · 1497 bp

Back to Staphylea

Pi 0.0114

MarkerSeek score 63.20

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1497
variable_sites: 32
pi: 0.0114
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1569-1586 bp	1.0	80.4
rps16-trnQ_p2	CCACATCGCTTCAAACGAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1549-1566 bp	1.0	79.6
rps16-trnQ_p3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1584-1601 bp	1.0	78.5
rps16-trnQ_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1583-1600 bp	1.0	78.5
rps16-trnQ_p5	ACCACATCGCTTCAAACGAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	1550-1567 bp	1.0	78.2