Hotspot

trnH-GUG-psbA

LSC · 173-628 · 456 bp

Back to Staphylea

Pi 0.0102

MarkerSeek score 57.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 9
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTAGCTGTTGTCGAAGCTCC	55.0	55.0	58.1	58.4	508-529 bp	1.0	79.8
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	569-590 bp	1.0	79.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	583-604 bp	1.0	79.5
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	582-603 bp	1.0	79.5
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	584-605 bp	1.0	79.5