Hotspot

ycf1

SSC · 108298-113601 · 5304 bp

Back to Staehelina

Pi 0.00748

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5304
variable_sites: 59
pi: 0.00748
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACCGACTCCGAAAGTTGTT	TTGAGCGCGTCCTTAAAAGA	50.0	45.0	58.1	57.8	385-439 bp	1.0	79.8
ycf1_p2	GACCGACTCCGAAAGTTGTT	ACCCGGGCAGGTTCTATAAT	50.0	50.0	58.1	58.2	631-685 bp	1.0	79.0
ycf1_p3	GACCGACTCCGAAAGTTGTT	CCCGGGCAGGTTCTATAATG	50.0	55.0	58.1	57.8	630-684 bp	1.0	79.0
ycf1_p4	GACCGACTCCGAAAGTTGTT	GACGCAAACGCTTTGTCATT	50.0	45.0	58.1	58.2	582-636 bp	1.0	78.6
ycf1_p5	GACCGACTCCGAAAGTTGTT	CAGGAGACCATATACCGGGA	50.0	55.0	58.1	57.7	494-548 bp	1.0	78.5