Hotspot

trnT-GGU

LSC · 30735-30806 · 72 bp

Back to Staehelina

Pi 0.0185

MarkerSeek score 64.30

Primer available yes

Label trnT-GGU

Feature Metadata

label_name: trnT-GGU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 2
pi: 0.0185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU_p1	GTGACCCGTTCAGGAATTCA	CGCTATTCTTCTTTCAAATACTACGA	50.0	34.6	57.8	57.7	142-144 bp	1.0	41.1
trnT-GGU_p2	GTGACCCGTTCAGGAATTCA	TCGCTATTCTTCTTTCAAATACTACG	50.0	34.6	57.8	57.7	143-145 bp	1.0	41.1
trnT-GGU_p3	TGACCCGTTCAGGAATTCAC	CGCTATTCTTCTTTCAAATACTACGA	50.0	34.6	57.8	57.7	141-143 bp	1.0	41.1
trnT-GGU_p4	TGACCCGTTCAGGAATTCAC	TCGCTATTCTTCTTTCAAATACTACG	50.0	34.6	57.8	57.7	142-144 bp	1.0	41.1
trnT-GGU_p5	CAGTGACCCGTTCAGGAATT	CGCTATTCTTCTTTCAAATACTACGA	50.0	34.6	57.8	57.7	144-146 bp	1.0	41.1