Hotspot

rbcL

LSC · 54765-56192 · 1428 bp

Back to Staehelina

Pi 0.00373

MarkerSeek score 61.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 8
pi: 0.00373
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TATGCATGTTGGATTCGGCT	50.0	45.0	57.1	57.7	1562 bp	1.0	70.1
rbcL_p2	ACGCCTAATTCATGTCGAGT	TATGCATGTTGGATTCGGCT	45.0	45.0	57.0	57.7	1610 bp	1.0	69.9
rbcL_p3	TTCATGAGTTGTAGGGAGGGA	TATGCATGTTGGATTCGGCT	47.6	45.0	57.8	57.7	1563 bp	1.0	68.0
rbcL_p4	AACGCCTAATTCATGTCGAGT	TATGCATGTTGGATTCGGCT	42.9	45.0	57.8	57.7	1611 bp	1.0	67.6
rbcL_p5	TCATGAGTTGTAGGGAGGGAT	TATGCATGTTGGATTCGGCT	47.6	45.0	57.6	57.7	1562 bp	1.0	66.3