Hotspot

rpl32-ndhF

SSC · 123713-124750 · 1038 bp

Back to Staehelina

Pi 0.00899

MarkerSeek score 50.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1038
variable_sites: 14
pi: 0.00899
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGCGTGTCTACCGATTTCAC	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.0	58.0	1731-1747 bp	0.666667	67.0
rpl32-ndhF_p2	CAGCGTGTCTACCGATTTCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	58.0	58.0	1732-1748 bp	0.666667	67.0
rpl32-ndhF_p3	AATAAGACTCCCCACCACCA	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.9	58.0	1554-1570 bp	0.666667	66.7
rpl32-ndhF_p4	AGCGTGTCTACCGATTTCAC	AACAGGTCGTGTGAACCAAA	50.0	45.0	58.0	57.9	2143-2159 bp	0.666667	66.2
rpl32-ndhF_p5	AGCGTGTCTACCGATTTCAC	ACAGGTCGTGTGAACCAAAA	50.0	45.0	58.0	57.9	2142-2158 bp	0.666667	66.2