Hotspot

petA-psbJ

LSC · 62684-63476 · 793 bp

Back to Staehelina

Pi 0.00421

MarkerSeek score 61.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 5
pi: 0.00421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	CCGGATTGGGTTCATCTCTG	45.0	55.0	58.0	58.0	928-938 bp	1.0	79.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.9	58.0	918-928 bp	1.0	79.8
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	ATCGGTTTAGTGGGCGTTTT	45.0	45.0	58.0	58.1	965-975 bp	1.0	79.3
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ATCGGTTTAGTGGGCGTTTT	50.0	45.0	57.9	58.1	955-965 bp	1.0	79.3
petA-psbJ_p5	ACGTGTACAAGGCCTTTTGT	TCCGGATTGGGTTCATCTCT	45.0	50.0	58.0	58.1	929-939 bp	1.0	79.2