Hotspot

accD-psaI

LSC · 58212-58883 · 672 bp

Back to Staehelina

Pi 0.00704

MarkerSeek score 53.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 7
pi: 0.00704
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	ACCAAAACAGAGGGAAAGTT	47.6	40.0	57.2	55.0	807-808 bp	1.0	43.9
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	ACCAAAACAGAGGGAAAGTT	47.6	40.0	57.2	55.0	806-807 bp	1.0	43.9
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	ACCAAAACAGAGGGAAAGTT	45.0	40.0	56.2	55.0	808-809 bp	1.0	43.9
accD-psaI_p4	TCCAGAAGGGCTTATTCGAT	ACCAAAACAGAGGGAAAGTT	45.0	40.0	56.0	55.0	807-808 bp	1.0	42.3
accD-psaI_p5	TTCCAGAAGGGCTTATTCGATC	ACCAAAACAGAGGGAAAGTT	45.5	40.0	57.9	55.0	808-809 bp	1.0	42.3