Hotspot

psaA-ycf3

LSC · 40991-41724 · 734 bp

Back to Staehelina

Pi 0.00454

MarkerSeek score 61.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 5
pi: 0.00454
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	TGAAGCGCATAACTGGTTGA	55.0	45.0	57.8	57.8	875-876 bp	1.0	77.2
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	926-927 bp	1.0	76.5
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	928-929 bp	1.0	76.5
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	TGAAGCGCATAACTGGTTGA	50.0	45.0	58.5	57.8	810-811 bp	1.0	75.2
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AGCGCATAACTGGTTGAAGA	55.0	45.0	57.8	57.5	872-873 bp	1.0	74.9