Hotspot

atpI-atpH

LSC · 24804-25939 · 1136 bp

Back to Staehelina

Pi 0.0047

MarkerSeek score 61.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 8
pi: 0.0047
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1271-1273 bp	1.0	78.2
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1327-1329 bp	1.0	77.6
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1287-1289 bp	1.0	77.3
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1272-1274 bp	1.0	77.0
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1331-1333 bp	1.0	76.9