Hotspot

trnE-UUC-rpoB

LSC · 11802-12618 · 817 bp

Back to Staehelina

Pi 0.00571

MarkerSeek score 63.10

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 7
pi: 0.00571
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	CCTCCTTGTCTCCGTGAAAG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	57.9	56.8	945-961 bp	1.0	62.6
trnE-UUC-rpoB_p2	GGACTTGAACCCTCCTTGTC	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	57.8	56.8	955-971 bp	1.0	61.8
trnE-UUC-rpoB_p3	TCGTCAACCCGATAGACCAT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	918-934 bp	1.0	61.5
trnE-UUC-rpoB_p4	CCTCCTTGTCTCCGTGAAAG	TCAAACCCTGATCAATGAACCT	55.0	40.9	57.9	57.6	935-951 bp	1.0	61.2
trnE-UUC-rpoB_p5	CCTCCTTGTCTCCGTGAAAG	TCTTCTGTCAAACCCTGATCA	55.0	42.9	57.9	56.6	942-958 bp	1.0	60.9