Hotspot

trnK-UUU-rps16

LSC · 4362-5086 · 725 bp

Back to Staehelina

Pi 0.00748
MarkerSeek score 65.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
725
variable_sites
8
pi
0.00748
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 922-997 bp 1.0 78.6
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 861-936 bp 1.0 78.0
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 860-935 bp 1.0 78.0
trnK-UUU-rps16_p4 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 876-951 bp 1.0 77.2
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 870-945 bp 1.0 77.1