Hotspot

ycf1

SSC · 131804-137443 · 5640 bp

Back to Stachyurus

Pi 0.0017

MarkerSeek score 35.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 17
pi: 0.0017
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATGATATAGAAAGATCCCCT	TCTCACAATTCATCAAGACCA	39.1	38.1	55.9	54.9	263 bp	1.0	41.5
ycf1_p2	TCGATGATATAGAAAGATCCCCT	TCTCACAATTCATCAAGACCAA	39.1	36.4	55.9	55.6	263 bp	1.0	41.5
ycf1_p3	TCGATGATATAGAAAGATCCCCTT	TCTCACAATTCATCAAGACCA	37.5	38.1	56.6	54.9	263 bp	1.0	41.5
ycf1_p4	TTCGATGATATAGAAAGATCCCCT	TCTCACAATTCATCAAGACCA	37.5	38.1	56.6	54.9	264 bp	1.0	41.5
ycf1_p5	TCGATGATATAGAAAGATCCCCT	ATCTCACAATTCATCAAGACCA	39.1	36.4	55.9	55.4	264 bp	1.0	41.5