Hotspot

rpl16

LSC · 87788-89307 · 1520 bp

Back to Stachyurus

Pi 0.00456

MarkerSeek score 57.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1520
variable_sites: 11
pi: 0.00456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCAAAGCAAAAGAGACGAAG	TGAGCAATTCTATAGGGTTGA	42.9	38.1	57.7	54.0	1661-1694 bp	1.0	42.6
rpl16_p2	TGCAAAGCAAAAGAGACGAAG	AAGGCAAACTCTTTCATTCT	42.9	35.0	57.7	53.0	1710-1743 bp	1.0	42.6
rpl16_p3	TGCAAAGCAAAAGAGACGAAG	AGGCAAACTCTTTCATTCTT	42.9	35.0	57.7	53.0	1709-1742 bp	1.0	42.6
rpl16_p4	TGCAAAGCAAAAGAGACGAAG	AAGGCAAACTCTTTCATTCTT	42.9	33.3	57.7	53.9	1710-1743 bp	1.0	42.6
rpl16_p5	TGCAAAGCAAAAGAGACGAAG	AGGCAAACTCTTTCATTCTTT	42.9	33.3	57.7	53.9	1709-1742 bp	1.0	42.6