Hotspot

ndhF

SSC · 117420-119660 · 2241 bp

Back to Stachyurus

Pi 0.00232

MarkerSeek score 30.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 9
pi: 0.00232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2771-2796 bp	1.0	79.8
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2647-2672 bp	1.0	79.8
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2639-2664 bp	1.0	79.8
ndhF_p4	AGGGGGACTAAACAGGAACA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	57.9	57.9	2658-2683 bp	1.0	79.2
ndhF_p5	GCAGAATACCGTCACCCATT	GAGCCGGTGAATCAGAAACA	50.0	50.0	58.0	58.2	2770-2795 bp	1.0	79.1