Hotspot

ccsA-ndhD

SSC · 123163-123435 · 273 bp

Back to Stachyurus

Pi 0.0256

MarkerSeek score 70.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 273
variable_sites: 12
pi: 0.0256
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTGGCTTCTA	TCCGGATTTCGTTCTTTCACT	45.0	42.9	57.8	57.6	451-453 bp	1.0	69.6
ccsA-ndhD_p2	AATTCCGCAATTGTGGCTTC	TCCGGATTTCGTTCTTTCACT	45.0	42.9	57.6	57.6	453-455 bp	1.0	68.2
ccsA-ndhD_p3	TCCGCAATTGTGGCTTCTAT	TCCGGATTTCGTTCTTTCACT	45.0	42.9	57.6	57.6	450-452 bp	1.0	67.8
ccsA-ndhD_p4	ATTCCGCAATTGTGGCTTCT	TCCGGATTTCGTTCTTTCACT	45.0	42.9	58.5	57.6	452-454 bp	1.0	67.5
ccsA-ndhD_p5	TTCCGCAATTGTGGCTTCTA	TCCGGATTTCGTTCTTTCAC	45.0	45.0	57.8	56.1	451-453 bp	1.0	65.5