Hotspot

ndhF-rpl32

SSC · 119661-120794 · 1134 bp

Back to Stachyurus

Pi 0.000883

MarkerSeek score 54.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1134
variable_sites: 2
pi: 0.000883
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	1326-1345 bp	1.0	76.7
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	AAAAAGCTTTCAACGCTGCC	45.0	45.0	57.8	58.4	1328-1347 bp	1.0	75.5
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	TTCAACGCTGCCCAATATCC	45.0	50.0	58.0	58.6	1318-1337 bp	1.0	75.2
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	AAAAAGCTTTCAACGCTGCC	50.0	45.0	57.7	58.4	1279-1298 bp	1.0	75.0
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTCAACGCTGCCCAATATCC	45.0	50.0	57.8	58.6	1320-1339 bp	1.0	74.0