Hotspot

infA-rps8

LSC · 86561-86687 · 127 bp

Back to Stachyurus

Pi 0.011

MarkerSeek score 63.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 2
pi: 0.011
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AACCCGGATCATACCATTGG	GCGGGATGGGGATTGTAATT	50.0	50.0	57.3	58.0	286-303 bp	1.0	76.6
infA-rps8_p2	CCCGGATCATACCATTGGGA	GCGGGATGGGGATTGTAATT	55.0	50.0	58.9	58.0	284-301 bp	1.0	74.4
infA-rps8_p3	CCGGATCATACCATTGGGAA	GCGGGATGGGGATTGTAATT	50.0	50.0	57.1	58.0	283-300 bp	1.0	74.3
infA-rps8_p4	AACCCGGATCATACCATTGG	TCGGCGGAGAAATTTTGTGT	50.0	45.0	57.3	58.4	214-231 bp	1.0	74.1
infA-rps8_p5	AACCCGGATCATACCATTGG	AGAATTTTAGGCGGGATGGG	50.0	50.0	57.3	57.6	296-313 bp	1.0	73.7