Hotspot

rps4-trnT-UGU

LSC · 50317-50725 · 409 bp

Back to Stachyurus

Pi 0.00733

MarkerSeek score 57.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 5
pi: 0.00733
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GGGGCGTGATTGGTTTCTAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	578-604 bp	1.0	82.1
rps4-trnT-UGU_p2	GGGGCGTGATTGGTTTCTAA	GCATTTGTAATGCGACGGTC	50.0	50.0	58.2	58.2	560-586 bp	1.0	81.7
rps4-trnT-UGU_p3	GATCGCTTACGGATCTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	558-584 bp	1.0	81.5
rps4-trnT-UGU_p4	GATCGCTTACGGATCTAGGC	GCATTTGTAATGCGACGGTC	55.0	50.0	57.7	58.2	540-566 bp	1.0	81.1
rps4-trnT-UGU_p5	GGGGCGTGATTGGTTTCTAA	CGGTTATTTGAGCCCGCTTA	50.0	50.0	58.2	58.3	599-625 bp	1.0	80.2