Hotspot

trnG-UCC-trnfM-CAU

LSC · 40270-40510 · 241 bp

Back to Stachyurus

Pi 0.0243

MarkerSeek score 69.90

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 10
pi: 0.0243
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	288-319 bp	1.0	85.0
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	286-317 bp	1.0	83.9
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	309-340 bp	1.0	83.3
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	308-339 bp	1.0	83.3
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TCTATTCATCGCGGGGTAGA	50.0	50.0	57.8	57.7	337-368 bp	1.0	82.7