Hotspot

psbZ-trnG-UCC

LSC · 39766-40198 · 433 bp

Back to Stachyurus

Pi 0.0122

MarkerSeek score 61.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 10
pi: 0.0122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	579-675 bp	1.0	83.4
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	582-678 bp	1.0	81.8
psbZ-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	575-671 bp	1.0	81.7
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	580-676 bp	1.0	81.4
psbZ-trnG-UCC_p5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	578-674 bp	1.0	80.6