Hotspot

trnC-GCA-petN

LSC · 29715-30711 · 997 bp

Back to Stachyurus

Pi 0.00201

MarkerSeek score 57.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 4
pi: 0.00201
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1031-1080 bp	1.0	80.0
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1006-1055 bp	1.0	79.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	1092-1141 bp	1.0	78.2
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	1067-1116 bp	1.0	77.3
trnC-GCA-petN_p5	TTCAAATCTGGGTGTCGCC	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	1004-1053 bp	1.0	74.4