Hotspot

rps16-trnQ-UUG

LSC · 6590-7742 · 1153 bp

Back to Stachyurus

Pi 0.000867

MarkerSeek score 52.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 2
pi: 0.000867
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1216-1230 bp	1.0	79.1
rps16-trnQ-UUG_p2	CCACATCGCTTCAAACGAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1196-1210 bp	1.0	78.4
rps16-trnQ-UUG_p3	GGCGGATGTAAGAATCCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1252-1266 bp	1.0	77.6
rps16-trnQ-UUG_p4	TGGCGGATGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1253-1267 bp	1.0	77.6
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1230-1244 bp	1.0	77.3