Hotspot

ycf1

SSC · 119633-124954 · 5322 bp

Back to Stachys

Pi 0.0187

MarkerSeek score 56.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5322
variable_sites: 248
pi: 0.0187
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCCAAGGTTTTTGGCAATT	CTCGAAACACGGACTCGATT	45.0	50.0	58.0	58.0	80-1096 bp	1.0	85.0
ycf1_p2	TTTGCCCCTTCTTGAACGAA	CTCGAAACACGGACTCGATT	45.0	50.0	57.9	58.0	80-585 bp	0.9	80.8
ycf1_p3	AAAAGGGGAGTTCGGTTTCC	CTCGAAACACGGACTCGATT	50.0	50.0	58.0	58.0	80-1390 bp	0.8	75.9
ycf1_p4	GAACAAAAGGGGAGTTCGGT	CTCGAAACACGGACTCGATT	50.0	50.0	58.0	58.0	80-1394 bp	0.8	75.8
ycf1_p5	TGAACAAAAGGGGAGTTCGG	CTCGAAACACGGACTCGATT	50.0	50.0	58.0	58.0	80-1395 bp	0.8	75.8