Hotspot

ndhF

SSC · 106778-109006 · 2229 bp

Back to Stachys

Pi 0.0152

MarkerSeek score 50.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 83
pi: 0.0152
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	80-2523 bp	1.0	82.8
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	80-2522 bp	1.0	82.8
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	80-2514 bp	1.0	82.7
ndhF_p4	TGACAAATTCTACGGCTCGG	AAAGAGGTAAGGTCCGGTGA	50.0	50.0	58.0	58.0	80-2716 bp	0.8	74.4
ndhF_p5	TGACAAATTCTACGGCTCGG	AAGAGGTAAGGTCCGGTGAA	50.0	50.0	58.0	58.0	80-2715 bp	0.8	74.4