Hotspot

rpl32-trnL-UAG

SSC · 109626-110127 · 502 bp

Back to Stachys

Pi 0.0252

MarkerSeek score 61.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 502
variable_sites: 33
pi: 0.0252
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCACCGGACCTTACCTCTTT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	80-1400 bp	1.0	87.7
rpl32-trnL-UAG_p2	TTCACCGGACCTTACCTCTT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	80-1401 bp	1.0	87.7
rpl32-trnL-UAG_p3	TCACCGGACCTTACCTCTTT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	80-1401 bp	1.0	87.7
rpl32-trnL-UAG_p4	TTCACCGGACCTTACCTCTT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	80-1402 bp	1.0	87.7
rpl32-trnL-UAG_p5	TCACCGGACCTTACCTCTTT	TGATCCACATAAAAGGGCCG	50.0	50.0	58.0	57.9	80-2000 bp	1.0	86.4