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psbT-psbN

LSC · 71460-71532 · 73 bp

Back to Stachys

Pi 0.046

MarkerSeek score 71.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 8
pi: 0.046
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	80-133 bp	1.0	70.4
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	80-181 bp	1.0	70.3
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	80-189 bp	1.0	69.4
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	80-192 bp	1.0	69.3
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	47.6	55.0	57.6	57.5	80-182 bp	1.0	68.2