Hotspot

rpl20-rps12

LSC · 66373-67105 · 733 bp

Back to Stachys

Pi 0.0199

MarkerSeek score 55.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 33
pi: 0.0199
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGGGCTATATATCCGCGTTT	CGAGGAACATGTACTAGGGTG	50.0	52.4	57.7	57.6	80-799 bp	1.0	70.4
rpl20-rps12_p2	TCCGCGTTTAATTCTGGTCA	CGAGGAACATGTACTAGGGTG	45.0	52.4	57.5	57.6	80-788 bp	1.0	69.1
rpl20-rps12_p3	TTTTTGTTCTACGCCTCCGG	CGAGGAACATGTACTAGGGTG	50.0	52.4	58.5	57.6	80-816 bp	1.0	68.7
rpl20-rps12_p4	CGGGCTATATATCCGCGTTT	CGAGGAACATGTACTAGGGT	50.0	50.0	57.7	56.1	80-799 bp	1.0	66.4
rpl20-rps12_p5	CCCCGAAAACTTGATGCAAA	CGAGGAACATGTACTAGGGTG	45.0	52.4	57.2	57.6	80-844 bp	1.0	66.1