Hotspot

petN-psbM

LSC · 28399-28906 · 508 bp

Back to Stachys

Pi 0.019

MarkerSeek score 63.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 24
pi: 0.019
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	80-595 bp	1.0	83.6
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	50.0	50.0	58.0	58.4	80-593 bp	1.0	83.6
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	55.0	50.0	57.9	58.4	80-592 bp	1.0	83.1
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	80-592 bp	1.0	82.2
petN-psbM_p5	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	50.0	50.0	58.0	57.4	80-594 bp	1.0	82.1