Hotspot

rps16-trnQ-UUG

LSC · 5794-6540 · 747 bp

Back to Stachys

Pi 0.0197

MarkerSeek score 64.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 37
pi: 0.0197
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	80-976 bp	1.0	86.8
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	80-918 bp	1.0	86.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	80-959 bp	1.0	86.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	80-901 bp	1.0	86.1
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	80-977 bp	1.0	85.8