Hotspot

psaC-ndhE

SSC · 123670-124567 · 898 bp

Back to Stachyphrynium

Pi 0.0777

MarkerSeek score 78.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 898
variable_sites: 61
pi: 0.0777
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GAGTACGGGCTTTTGTCGAT	CCAGATCATCGAACAGGACC	50.0	55.0	58.0	57.8	1889-1896 bp	1.0	85.4
psaC-ndhE_p2	ATACATCTGTAGGGCAGGCT	CCAGATCATCGAACAGGACC	50.0	55.0	57.9	57.8	1657-1658 bp	1.0	84.8
psaC-ndhE_p3	TCGATTCCCAGTCTCCAGTT	CCAGATCATCGAACAGGACC	50.0	55.0	58.1	57.8	2201 bp	0.5	57.7
psaC-ndhE_p4	TACAACCCACACAGTCCTCT	CCAGATCATCGAACAGGACC	50.0	55.0	57.9	57.8	1735 bp	0.5	57.4
psaC-ndhE_p5	TTTGACCGCTGTACATTGCT	CCAGATCATCGAACAGGACC	45.0	55.0	58.1	57.8	2329 bp	0.5	57.3