Hotspot

psbT-psbN

LSC · 78796-78860 · 65 bp

Back to Stachyphrynium

Pi 0.0615

MarkerSeek score 72.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 4
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTTCGAGAGCCGCCTAAG	ATCCATTCGAAGAACACGGG	50.0	50.0	58.3	58.0	135 bp	1.0	80.0
psbT-psbN_p2	TTTTTCGAGAGCCGCCTAAG	GGGTACGCCTTATATACCGC	50.0	55.0	58.3	57.7	184 bp	1.0	77.2
psbT-psbN_p3	TTTTTCGAGAGCCGCCTAAG	TTCGAAGAACACGGGGACTA	50.0	50.0	58.3	58.4	130 bp	1.0	77.2
psbT-psbN_p4	CCGCCTAAGGTTCCAACTAA	ATCCATTCGAAGAACACGGG	50.0	50.0	57.2	58.0	124 bp	1.0	76.2
psbT-psbN_p5	TTTTTCGAGAGCCGCCTAAG	GGCAACCCTCTCAACAACTA	50.0	50.0	58.3	57.4	159 bp	1.0	75.3