Hotspot

psaJ-rpl33

LSC · 71526-71913 · 388 bp

Back to Stachyphrynium

Pi 0.0398

MarkerSeek score 71.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 15
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATCGTTTATTCCCGGATGCC	GGCACAACTGGTACATTCCA	50.0	50.0	58.1	58.1	478-486 bp	1.0	82.7
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	GGCACAACTGGTACATTCCA	50.0	50.0	57.9	58.1	464-472 bp	1.0	82.5
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	GGCACAACTGGTACATTCCA	50.0	50.0	57.7	58.1	515-523 bp	1.0	81.0
psaJ-rpl33_p4	CGTTTATTCCCGGATGCCTT	GGCACAACTGGTACATTCCA	50.0	50.0	58.3	58.1	476-484 bp	1.0	81.0
psaJ-rpl33_p5	TTATTCCCGGATGCCTTGTC	GGCACAACTGGTACATTCCA	50.0	50.0	57.7	58.1	473-481 bp	1.0	80.9