Hotspot

trnE-UUC-trnT-GGU

LSC · 33298-34253 · 956 bp

Back to Stachyphrynium

Pi 0.057

MarkerSeek score 83.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 51
pi: 0.057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1043-1054 bp	1.0	86.7
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1041-1052 bp	1.0	86.7
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1095-1106 bp	1.0	86.5
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	1020-1031 bp	1.0	86.3
trnE-UUC-trnT-GGU_p5	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	1074-1085 bp	1.0	86.1