Hotspot

petN-psbM

LSC · 30284-31436 · 1153 bp

Back to Stachyphrynium

Pi 0.0351

MarkerSeek score 74.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 39
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTTTTCATTCT	55.0	38.1	58.1	55.8	1257-1438 bp	1.0	58.3
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTTTTCATTCT	55.0	38.1	58.1	55.8	1256-1437 bp	1.0	58.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTTTTCATTCT	50.0	38.1	58.2	55.8	1315-1496 bp	1.0	57.9
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTTTTCATTCT	55.0	38.1	57.8	55.8	1255-1436 bp	1.0	57.9
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTTTTCATTCT	60.0	38.1	58.2	55.8	1252-1433 bp	1.0	57.6