Hotspot

atpH-atpI

LSC · 15080-15962 · 883 bp

Back to Stachyphrynium

Pi 0.0585

MarkerSeek score 79.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 50
pi: 0.0585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GGTGAATCCATGGAAGGTCA	45.0	50.0	58.0	57.2	942-989 bp	1.0	79.7
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	GGTGAATCCATGGAAGGTCA	50.0	50.0	58.0	57.2	1025-1072 bp	1.0	79.1
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	GGTGAATCCATGGAAGGTCA	50.0	50.0	57.9	57.2	998-1045 bp	1.0	78.8
atpH-atpI_p4	AATAGAAGCAAGCCCCACAG	GGTGAATCCATGGAAGGTCA	50.0	50.0	57.9	57.2	976-1023 bp	1.0	78.7
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	GGTGAATCCATGGAAGGTCA	45.0	50.0	57.9	57.2	958-1005 bp	1.0	78.7