Hotspot

trnQ-UUG-psbK

LSC · 7432-7863 · 432 bp

Back to Stachyphrynium

Pi 0.0576

MarkerSeek score 78.50

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 24
pi: 0.0576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAAGGATTCGAACCTCCGAG	GCTTCAGGCAATTTGGCAAA	55.0	45.0	57.8	58.1	582-596 bp	1.0	82.7
trnQ-UUG-psbK_p2	AGGATTCGAACCTCCGAGTA	GCTTCAGGCAATTTGGCAAA	50.0	45.0	57.6	58.1	580-594 bp	1.0	80.9
trnQ-UUG-psbK_p3	AAGGATTCGAACCTCCGAGT	GCTTCAGGCAATTTGGCAAA	50.0	45.0	58.4	58.1	581-595 bp	1.0	80.8
trnQ-UUG-psbK_p4	GAGTAACGGGACCAAAACCC	GCTTCAGGCAATTTGGCAAA	55.0	45.0	58.5	58.1	565-579 bp	1.0	80.7
trnQ-UUG-psbK_p5	ATTCGAACCTCCGAGTAACG	GCTTCAGGCAATTTGGCAAA	50.0	45.0	57.4	58.1	577-591 bp	1.0	79.9