Hotspot

rps16-trnQ-UUG

LSC · 6220-7359 · 1140 bp

Back to Stachyphrynium

Pi 0.0347
MarkerSeek score 70.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1140
variable_sites
39
pi
0.0347
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1215-1231 bp 1.0 82.8
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1213-1229 bp 1.0 82.8
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1221-1237 bp 1.0 82.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1197-1213 bp 1.0 81.9
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1219-1235 bp 1.0 81.7