Hotspot

ycf3

LSC · 45405-47483 · 2079 bp

Back to Spyridium

Pi 0.00167

MarkerSeek score 60.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2079
variable_sites: 25
pi: 0.00167
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATTGGTTCTTCCCAGAGA	TTTCTCACAACACGAAAGGT	45.0	40.0	55.8	55.4	80-2631 bp	1.0	46.2
ycf3_p2	TGATTGGTTCTTCCCAGAGAA	TTTCTCACAACACGAAAGGT	42.9	40.0	56.6	55.4	80-2287 bp	1.0	44.6
ycf3_p3	TTGATTGGTTCTTCCCAGAGA	TTTCTCACAACACGAAAGGT	42.9	40.0	56.6	55.4	80-2632 bp	1.0	44.6
ycf3_p4	TGATTGGTTCTTCCCAGAGA	TTTTCTCACAACACGAAAGGT	45.0	38.1	55.8	56.2	80-2632 bp	1.0	44.6
ycf3_p5	TGGTTCTTCCCAGAGAAATGA	TTTCTCACAACACGAAAGGT	42.9	40.0	56.6	55.4	80-2283 bp	0.954545	42.7