Hotspot

ycf1

SSC · 130036-135744 · 5709 bp

Back to Spyridium

Pi 0.00398

MarkerSeek score 49.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5709
variable_sites: 173
pi: 0.00398
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGACGAAAATCCGGTTGTTG	TCCCTTCTTGAATGGTCCCT	50.0	50.0	58.1	57.9	80-645 bp	1.0	84.5
ycf1_p2	ATGTCTCGACGAAAATCCGG	TCCCTTCTTGAATGGTCCCT	50.0	50.0	58.1	57.9	80-651 bp	1.0	84.4
ycf1_p3	CTCGGACCATTCGGGAAATT	TCCCTTCTTGAATGGTCCCT	50.0	50.0	58.0	57.9	80-1090 bp	1.0	83.9
ycf1_p4	AGCGCGCATAGAACCTTTTA	TCCCTTCTTGAATGGTCCCT	45.0	50.0	58.0	57.9	80-673 bp	0.954545	82.6
ycf1_p5	AGCGCGCATAGAACCTTTTA	TTACATACCCGCCCAGTTTG	45.0	50.0	58.0	57.9	80-947 bp	0.954545	82.4