Hotspot

ndhF

SSC · 115514-117760 · 2247 bp

Back to Spyridium

Pi 0.00244

MarkerSeek score 39.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 55
pi: 0.00244
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AAGCCGGTGAATCAGAAACA	50.0	45.0	58.0	57.7	80-2908 bp	1.0	83.1
ndhF_p2	GCAGAATACCGTCACCCATT	AGCCGGTGAATCAGAAACAA	50.0	45.0	58.0	57.7	80-2907 bp	1.0	83.1
ndhF_p3	AGGGGGACTAAACAGGAACA	AAGCCGGTGAATCAGAAACA	50.0	45.0	57.9	57.7	80-2795 bp	1.0	82.6
ndhF_p4	AGGGGGACTAAACAGGAACA	AGCCGGTGAATCAGAAACAA	50.0	45.0	57.9	57.7	80-2794 bp	1.0	82.6
ndhF_p5	ACAACTTCAGAAACGAGGGG	AGCCGGTGAATCAGAAACAA	50.0	45.0	57.7	57.7	80-2809 bp	1.0	81.5