Hotspot

ccsA-ndhD

SSC · 121410-121678 · 269 bp

Back to Spyridium

Pi 0.0243

MarkerSeek score 56.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 23
pi: 0.0243
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAAATTCCGCAATTGTGGCC	TCAGTTGATAAAGTCGAAGCT	45.0	38.1	57.3	55.0	80-483 bp	1.0	52.3
ccsA-ndhD_p2	GGCCTCGATGGGATTTCTTA	TCAGTTGATAAAGTCGAAGCT	50.0	38.1	57.1	55.0	80-467 bp	1.0	50.2
ccsA-ndhD_p3	TGGCCTCGATGGGATTTCTT	TCAGTTGATAAAGTCGAAGCT	50.0	38.1	59.1	55.0	80-468 bp	1.0	49.7
ccsA-ndhD_p4	AAATTCCGCAATTGTGGCCT	TCAGTTGATAAAGTCGAAGCT	45.0	38.1	59.0	55.0	80-482 bp	1.0	49.6
ccsA-ndhD_p5	TAAATTCCGCAATTGTGGCC	ATCAGTTGATAAAGTCGAAGCT	45.0	36.4	57.3	55.6	80-484 bp	1.0	49.6