Hotspot

rpl32-trnL-UAG

SSC · 119065-120261 · 1197 bp

Back to Spyridium

Pi 0.00894

MarkerSeek score 68.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1197
variable_sites: 83
pi: 0.00894
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTCGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	80-1338 bp	1.0	88.9
rpl32-trnL-UAG_p2	AGGGATATTCGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	80-1339 bp	1.0	88.9
rpl32-trnL-UAG_p3	GGGATATTCGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	80-1337 bp	1.0	88.9
rpl32-trnL-UAG_p4	GGGATATTCGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	80-1338 bp	1.0	88.9
rpl32-trnL-UAG_p5	GGCAGCATTGAAAGCCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	80-1328 bp	1.0	88.4