Hotspot

ndhF-rpl32

SSC · 117761-118905 · 1145 bp

Back to Spyridium

Pi 0.00895

MarkerSeek score 67.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1145
variable_sites: 75
pi: 0.00895
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATAGGGGTTGGAAGGGGAAT	CAATGCTGCCGAATATCCCT	50.0	50.0	57.7	58.1	80-1426 bp	1.0	84.8
ndhF-rpl32_p2	ATAGGGGTTGGAAGGGGAAT	TCAATGCTGCCGAATATCCC	50.0	50.0	57.7	58.1	80-1427 bp	1.0	84.8
ndhF-rpl32_p3	GGGTTGGAAGGGGAATGAAA	CAATGCTGCCGAATATCCCT	50.0	50.0	57.7	58.1	80-1422 bp	1.0	84.7
ndhF-rpl32_p4	GGGTTGGAAGGGGAATGAAA	TCAATGCTGCCGAATATCCC	50.0	50.0	57.7	58.1	80-1423 bp	1.0	84.6
ndhF-rpl32_p5	GGGTTGGAAGGGGAATGAAA	AAAAGGCTTTCAATGCTGCC	50.0	45.0	57.7	57.8	80-1432 bp	1.0	84.0